Phenotype Image Detail

Image

Caption

Delayed proximal epithelial cell differentiation in lungs of Creb1^tm3.1Gsc/Creb1^tm3.1Gsc fetal mice. Tissue sections from the lung of E16.5 and E18.5 mutant and wild-type (WT) were immunostained for Foxj1 (A-D), CC10 (E-H), and calcitonin gene related peptide (CGRP) (I-L). At E16.5 Foxj1 positive cells were readily detected in conducting airway epithelium (A), but much less frequently in mutant lungs (C, arrowheads show two Foxj1-positive cells). Increased frequency of Foxj1-positive cells was detected in the lung of both E18.5 WT (B) and E18.5 mutant (D) mice. In WT lungs, Scgb1a1-positive cells were not detected at E16.5 (E,) but were common at E18.5 (F). In the lung of E18.5 mutant mice, Scgb1a1-positive cells were almost completely absent (H, arrowhead shows a solitary Scgb1a1-positive cell). Single CGRP-positive cells were detected at E16.5 in WT (I, arrowheads show CGRP-positive cells), but not mutant (K) lungs. At E18.5 Clusters of CGRP-positive cells were then detected in both WT and mutant lungs (J,L, arrowheads show CGRP-positive cell clusters). qPCR analysis of mRNA levels for Foxj1, Scgb1a1 and Calca in the lung of E17.5 mutant mice relative to WT (n = 4) (M). Error bars represent SEM. Asterisk (*) indicates p<0.05. White bars: Wild-type, Black bars: Creb1-/-. Scale bars: A-L, 50 um.

Copyright

This image is from Bird AD, PLoS One 2011;6(3):e17843, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:171712

Associated Alleles

Symbol	Name
Creb1^tm3.1Gsc	cAMP responsive element binding protein 1; targeted mutation 3.1, Gunther Schutz

Associated Genotypes

Allelic Composition	Genetic Background
Creb1^tm3.1Gsc/Creb1^tm3.1Gsc	involves: 129P2/OlaHsd * C57BL/6