Phenotype Image Detail

Image

Caption

Shortened and disorganized outer rod segments (OS) and swollen retinal pigment epithelium (RPE) in Mfsd2a^tm1Dls/Mfsd2a^tm1Dls (KO) mice. A, plastic sections of eyes of 4-month-old mice. Toluidine blue staining was carried out to visualize the layers, as indicated to the right of the figure. OS of mutant mice appear thinner and more disorganized, with some outer nuclear layer (ONL) loss, although the other retinal layers appear comparable with wild type (WT). Scale bar, 50 um. B, S.E. images from 4-month-old mice show the disorganization of the OS in mutant mice, and RPE appears swollen. Scale bar, 2 um. The middle panel shows that villi of WT RPE is in close contact with the OS (black arrow), but villi of the mutant appears collapsed and not interacting with OS (black arrow). Scale bar, 2 um. The far right panel is of the basal membrane of the RPE. Edema was seen at the basal infoldings of mutant RPE but not in WT RPE (yellow arrow). Scale bar, 1 um. D, H&E staining of eyes of P13 mice was carried out to visualize the layers, as indicated to the right of the figure. OS of mutant mice appear thinner, although other retinal layers appear comparable with WT. Scale bar, 50 um. WT, n = 4; KO, n = 4.

Copyright

This image is from Wong BH, J Biol Chem 2016 May 13;291(20):10501-14 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:234197

Associated Alleles

Symbol	Name
Mfsd2a^tm1Dls	MFSD2 lysolipid transporter A, lysophospholipid; targeted mutation 1, David L Silver

Associated Genotypes

Allelic Composition	Genetic Background
Mfsd2a^tm1Dls/Mfsd2a^tm1Dls	B6.Cg-Mfsd2a^tm1Dls