Phenotype Image Detail

Image

Caption

A, S.E. images from 3-month-old Mfsd2a^tm1Dls/Mfsd2a^tm1Dls (KO) mice show large gaps in the subretinal space (black arrow). Scale bar, 2 um. The middle panel shows a phagocytic cell (white arrow). Scale bar, 2 um. The far right panel shows an example of a phagocytic cell containing outer rod segment (OS) discs in an endosome (yellow arrow). Scale bar, 1 um. AM, apical membrane; BI, basal infoldings. B, immunolocalization of the microglia/macrophage marker Iba-1 was performed on eye sections from P14 mice. Iba-1 positive cells, which normally reside in the inner eye, were found in the subretinal space (yellow arrow). Scale bar, 50 um. C, immunolocalization of Iba-1 as done in B. Iba-1-positive cells appeared rounder and less ramified compared with WT, indicative of an activated state. Scale bar 50 um. WT, n = 5; KO, n = 5. GCL, ganglion cell layer; INL, inner nuclear layer.

Copyright

This image is from Wong BH, J Biol Chem 2016 May 13;291(20):10501-14 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:234197

Associated Alleles

Symbol	Name
Mfsd2a^tm1Dls	MFSD2 lysolipid transporter A, lysophospholipid; targeted mutation 1, David L Silver

Associated Genotypes

Allelic Composition	Genetic Background
Mfsd2a^tm1Dls/Mfsd2a^tm1Dls	B6.Cg-Mfsd2a^tm1Dls