Phenotype Image Detail

Image

Caption

(A) SC transverse filament protein 1 (SCP1) and SCP3 distribution in testis sections from wild-type (WT) and Kash5^{tm1b(KOMP)Wtsi}/Kash5^{tm1b(KOMP)Wtsi} (Kash5-null) mice. WT testes show consistent colocalization of SCP1 and SCP3 where most SCP3-positive strands are also SCP1 positive (i.e., the cells are in pachytene). In mutant testes, SCP1 vs. SCP3 colocalization is more variable. SCP3 tends to form aggregates in mutant spermatocytes (arrows). These can also be seen in B and C (arrows). (B) Spermatocyte spreads from WT and mutant littermates. SCP3 and SCP1 were localized using structured illumination microscopy (SIM). In WT pachytene spermatocytes sister chromosomes are paired as indicated by both SCP3 and SCP1 labeling. SIM reveals strings of SCP1 foci lying between easily resolvable SCP3 axial strands. In mutant spermatocytes SCP3 appears mainly in single axial strands. Only limited regions of paired SCP3 strands are seen (inset) that are weakly positive for SCP1. (C) Testis sections labeled with an anti-centromere CREST auto-antibody and anti-SCP3. In WT sections spermatocytes positive for both CREST and SCP3 are restricted to the periphery of the seminiferous tubules. In mutant specimens CREST- and SCP3-positive cells persist into the center of the tubules. Higher magnification (i and ii, bottom panels) reveals that the number of CREST-positive foci in mutant spermatocytes (34.80 +/- 0.33 SEM) (ii) is noticeably greater than that in WT cells (21.96 +/- 0.45) (i). This difference is statistically significant (P < 0.0001).

Copyright

This image is from Horn HF, J Cell Biol 2013 Sep 30;202(7):1023-39, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:201813

Associated Alleles

Symbol	Name
Kash5^tm1b(KOMP)Wtsi	KASH domain containing 5; targeted mutation 1b, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Kash5^tm1b(KOMP)Wtsi/Kash5^tm1b(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N