Phenotype Image Detail

Image

Caption

Telomeres associate with SUN1 in wild-type (WT) and Kash5^{tm1b(KOMP)Wtsi}/Kash5^{tm1b(KOMP)Wtsi} (Kash5-null) spermatocytes. (A) Spermatocyte spreads processed for telomere fluorescence in situ hybridization (Tel-FISH) and immunostained with anti-SUN1. In WT specimens 96.8 +/- 0.6% of telomeres are associated with SUN1 foci. In mutant spermatocytes the figure is lower at 55.8 +/- 2.0%. Nevertheless, telomeres can still associate with SUN1 in the absence of KASH5. (B) Spermatocyte spreads visualized by structured illumination microscopy (SIM). SUN1 exhibits the same ring-like structure as KASH5 at the tips of SCP3-positive axial strands in WT spermatocytes (insets). In mutant spermatocytes, SUN1 also localizes to the tips of SCP3-positive strands. However, the ring-like organization is replaced by seemingly unstructured foci (insets).

Copyright

This image is from Horn HF, J Cell Biol 2013 Sep 30;202(7):1023-39, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:201813

Associated Alleles

Symbol	Name
Kash5^tm1b(KOMP)Wtsi	KASH domain containing 5; targeted mutation 1b, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Kash5^tm1b(KOMP)Wtsi/Kash5^tm1b(KOMP)Wtsi	involves: C57BL/6J * C57BL/6N