Phenotype Image Detail

Image

Caption

Failed centriole duplication, maturation, and ciliogenesis in cerebellar granule neuron progenitors (CGNPs) and ependymal cells in the Cep120^tm1.1Blnw/Cep120^tm1.2Blnw Tg(Nes-cre)1Atp/0 mutant. (A-D) Sagittal brain sections of P14 wild-type (WT) and Cep120^tm1.1Blnw/Cep120^tm1.2BlnwTg(Nes-cre)1Atp/0 (Cep120^f/-;nes-cre) mice were coimmunostained for acetylated alpha-tubulin and Arl13b, cilia markers. The fourth ventricular choroid plexus is circled. Framed areas in panels A and B are enlarged in A'/A" and B'/B". Panels A" and B" show representative areas of ependyma near the fourth ventricle. Panels C and D show representative areas of granule neuron progenitors (CGNPs). Arrowheads indicate representative cilia. Note that cilia develop in the mutant choroid plexus, but not in ependymal cells and CGNPs. (G-L) Sagittal cerebellar sections of P14 WT and mutant mice were coimmunostained for gamma-tubulin and Cep120, Odf2, or Ta3, as shown. Note that very few centrioles are present in mutant CGNPs, relative to WT CGNPs. Arrowheads indicate one or two representative centrioles in each panel.

Copyright

This image is from Wu C, PLoS One 2014;9(9):e107943, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:220629

Associated Alleles

Symbol	Name
Cep120^tm1.1Blnw	centrosomal protein 120; targeted mutation 1.1, Baolin Wang
Cep120^tm1.2Blnw	centrosomal protein 120; targeted mutation 1.2, Baolin Wang
Tg(Nes-cre)1Atp	transgene insertion 1, Andreas Trumpp

Associated Genotypes

Allelic Composition	Genetic Background
Cep120^tm1.1Blnw/Cep120^tm1.2Blnw Tg(Nes-cre)1Atp/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N