Phenotype Image Detail

Image

Caption

Srrm4^{tm1b(EUCOMM)Wtsi}/Srrm4^{tm1b(EUCOMM)Wtsi} (delta7-8/delta7-8) mice display aberrant cortical layering and premature neurogenesis. A) Immunofluorescence microscopy using antibodies to Tbr1, Satb2, NeuN, and Pax6 to label deep layer VI, superficial layers II-V, post-mitotic neurons, and neural progenitors, respectively, on coronal sections of E18.5 embryonic brains. Bars, 50 um. (I-VI) Cortical layers I-VI, (CM) cortical mantle. Dashed white lines highlight ventral and dorsal cortical boundaries. (B-E) The number of Tbr1+ (B), layer II-V Satb2+ (C), NeuN+ (D), and Pax6+ (E) cells was quantified for three to five individuals per genotype and on three sections for each individual. These immunostaining experiments highlight an increase in the number of deep, early-born Tbr1+ neurons and a corresponding decrease in superficial Satb2+ neurons as well as a decrease in the total number of neurons (NeuN+) and neural progenitors (Pax6+). (F) EdU-labeling was performed at E12.5 (green), and brains were harvested at E18.5 and stained with an antibody to Tbr1 (red). Bar, 100 um. (G-I) The number of EdU+ cells was counted in deep layer VI (G), superficial layers II-V (H), and the SVZ(I). (J) The thickness of the SVZ was measured from the preplate to the lateral ventricle and relative to the total thickness of the cortex measured from the surface of layer I to the lateral ventricle. Four to five embryos per genotype and three sections per embryo were analyzed. Whiskers indicate the 10th and 90th percentiles in all box plots. One-way ANOVA with Tukey-Kramer post-hoc test.

Copyright

This image is from Quesnel-Vallieres M, Genes Dev 2015 Apr 1;29(7):746-59, and is displayed with the permission of Cold Spring Harbor Laboratory Press, who owns the Copyright. J:221584

Associated Alleles

Symbol	Name
Srrm4^tm1b(EUCOMM)Wtsi	serine/arginine repetitive matrix 4; targeted mutation 1b, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Srrm4^tm1b(EUCOMM)Wtsi/Srrm4^tm1b(EUCOMM)Wtsi	involves: C57BL/6J * C57BL/6N