Phenotype Image Detail

Image

Caption

Impaired cerebrospinal fluid (CSF) circulation in Ulk4^{tm1a(KOMP)Wtsi}/Ulk4^{tm1a(KOMP)Wtsi} mouse brain. Four P12 wild-type (WT, W1-W4) and three mutant (M1-M3) mice were intraventricularly injected with 5 ul of Evans blue dye into the dorsal cortex (4% in PBS). The dye was allowed to flow with the CSF in vivo for 20 min. Mouse brains were subsequently fixed, sectioned, and imaged at the comparable levels of LV (A), 3V (B), SCO (C), aqueduct (Aq; D), 4V (E), and spinal canal (SC; F ). Arrows indicate the anatomical positions of the LV, 3V, SCO, Aq, 4V, and SC of WT littermate and mutant brains. Note that in the WT brains, the dye flowed from the LV to SC with defined localization in the ventricles (W1-W4, A-F ). In three mutants, the dye was largely diffused from LV to 3V passively, with little dye reaching the SCO and no dye reaching the Aq, 4V, or SC of the mutant brain (D-F, bottom right), demonstrating an impaired CSF flow in the mutant mice. Scale bar, 600 um.

Copyright

This image is from Liu M, J Neurosci 2016 Jul 20;36(29):7589-600, an open-access article distributed under the terms of the Creative Commons Attribution-Noncommerical-NoDerivatives 4.0 International License. J:234631

Associated Alleles

Symbol	Name
Ulk4^tm1a(KOMP)Wtsi	unc-51-like kinase 4; targeted mutation 1a, Wellcome Trust Sanger Institute
Ulk4^tm1a(KOMP)Wtsi	unc-51-like kinase 4; targeted mutation 1a, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Ulk4^tm1a(KOMP)Wtsi/Ulk4^tm1a(KOMP)Wtsi	involves: C57BL/6N