Phenotype Image Detail

Image

Caption

Mitochondria accumulate in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} mice. Transmission electron-micrographs 1-months old wild-type (WT), heterozygous (HET) and Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) lenses. A. EM images of lens epithelium. The epithelium of KO lenses shows a number of double-membraned vesicles containing undegraded organelles, mostly mitochondria (shown by a black arrow, and also in the enlarged inset image). No such accumulation of mitochondria in the epithelium of WT or HET lenses was seen. B. EM images of the equatorial region of the lens. The fiber cells in KO lens show presence of mitochondria and other organelles as shown by red arrows and in enlarged inset image. C. EM images of the nuclear region (center) of the lens. The nuclear regions of WT and HET lenses were devoid of any cytoplasmic debris (indicated by arrows and in enlarged inset image). Cytoplasmic debris were present in the center of the KO lens (indicated by an arrow and enlarged inset image). n = 2 independent experiments. Scale bars: 2 um and inset images 500 nm.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N