Phenotype Image Detail

Image

Caption

Calcium level is altered in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} cells. Cultured lens epithelial cells (LECs) from wild-type (WT), and Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) lenses were incubated with a calcium- dye, Calcium Orange (4 uM; red) for 45 minutes, during which dye enters intracellular organelles. Fluorescence intensity was higher in KO cells than in the WT cells. Intense fluorescent signal was localized around the nucleus in KO (b) compared to diffused cytoplasmic staining in WT cells (a). (Data is representative of 8-10 cells for each type and n = 2 independent experiment). Scale bars: 20 um. Bar graph on the right is the quantification of calcium signal. *, P>0.05 by student's t test.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N