Phenotype Image Detail

Image

Caption

Integrity of cytoskeletal network is affected in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} lenses. Expression patterns of alpha-II spectrin in lens epithelial cells (LECs). Immunostaining of LECs with alpha-II spectrin antibody showing the cytoskeletal meshwork of alpha-spectrin in wild-type (WT) LECs. In contrast, in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) LECs the alpha-spectrins meshwork are aggregated. Data is representative of 6-8 cells for each type. Scale bars: 20 um. E. LECs were treated with F-actin phalloidin to visualize the actin polymerization. KO LECs showed a reduction in F-actin staining compared to WT LECs. n = 2 independent experiment. Scale bars: 20 um. G. Immunostaining of LECs with the alpha-tubulin antibody. WT and cells show well-spread tubulin strands compared to KO LECs. In KO cells tubulin strands are aggregated, and the aggregation is prominent near the edges (shown by arrow and inset panel; enlarged area). Data is representative of 6-8 cells for each type. Scale bars: 20 um.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N