Phenotype Image Detail

Image

Caption

Calpain-3 is activated in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) lenses. A. Immunostaining of 1-month old lens sections with anti-calpain Lp-85 antibody. KO lens show an increased expression of calpain compared to wild-type (WT). Inset images a and b are enlarged areas of the equatorial region of WT and KO lens sections respectively, demonstrating positive calpain staining in the nuclei in KO compared to WT lenses. B. Cultured lens epithelial cells (LECs) immunostained with anti-calpain Lp-85 antibody. KO cells clearly show calpain staining on the nucleus, compared to WT cells that show calpain staining around the nucleus. Inset images are enlarged areas to visualize the nuclear staining. Data is representative of 6-8 cells for each type. Scale bars: A&B: 50 um and a, b, c and inset images: 20 um.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N