Phenotype Image Detail

Image

Caption

Autophagic markers accumulate in Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) mice. B. Immunohistochemical staining of 1-month-old lens sections with the LC3 antibody. (LC3: red, DAPI nuclear stain: blue) Intensity of LC3 staining was increased, 2-3 fold in KO lenses compared to WT and HET lenses. The organelle free zone (OFZ) of KO lenses (panel c) showed positive LC3 staining compared to the corresponding region in wild-type (WT) (panel a) and HET (panel b) lenses. Bar graph on the right panel represent quantification of LC3 staining (mean S.D) in n = 4 lenses. *, P<0.05 by student's t test. C. Cultured Lens Epithelial Cells (LECs) from WT, Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1⁺ (HET) and Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (HOM) mice were examined by immunofluorescence using an anti-LC3 antibody (green). E. Immunohistochemical analysis of p62 distribution in mouse lens sections. LEC and lens fiber cells of the KO show increased p62 expression (red) and larger p62 aggregates compared to the LEC and lens fiber cells of WT and HET lens. The bar graph on the right shows the quantification of p62 intensity. Data represent mean S.D in n = 4 lenses. *, P<0.05 by student's t test. F. Immunofluorescence analysis of p62 in LEC. KO cells show increased expression of p62 and larger sized p62 aggregates compared to HET and WT cells. n = 2, independent experiments. H. Immunohistochemical analysis of ubiquitinated proteins in mouse lens sections. Outer cortical region of Cryba1^{tm1a(EUCOMM)Hmgu}/Cryba1^{tm1a(EUCOMM)Hmgu} (KO) showing drastic increase in ubiquitinated proteins (red) compared to control lenses. Bar graph on the right side is a quantification of ubiquitin signal represented as mean S.D in n = 3 lenses. *, P<0.05 by student's t test. Scale bars: B: 100 um, a,b and c, E, H: 50 um, and C, F:20 um.

Copyright

This image is from Hegde S, PLoS One 2016;11(2):e0149027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235696

Associated Alleles

Symbol	Name
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH
Cryba1^tm1a(EUCOMM)Hmgu	crystallin, beta A1; targeted mutation 1a, Helmholtz Zentrum Muenchen GmbH

Associated Genotypes

Allelic Composition	Genetic Background
Cryba1^tm1a(EUCOMM)Hmgu/Cryba1^tm1a(EUCOMM)Hmgu	involves: C57BL/6J * C57BL/6N