Phenotype Image Detail

Image

Caption

Abnormality in ductal branching in the Klf6-deficient prostate epithelium. Morphological ductal aberrations in Klf6-deficient anterior prostates were first observed at P5 and retained in adult mice. Light microscopy images (A, B, E, and F) and H&E staining (C and D) of Klf6^tm1Fjs/Klf6^tm1Fjs Nkx3-1⁺/Nkx3-1⁺ (Klf^f/fNks3.1^+/+; control) (A, C, and E) and Klf6^tm1Fjs/Klf6^tm1Fjs Nkx3-1^tm3(cre)Mms/Nkx3-1⁺ (Klf^f/fNkx3.1^Cre/+; mutant) (B, D, and F) from P5 and 1-year-old anterior prostates that were microdissected prior to imaging. Only one side of the symmetrical anterior lobe was imaged, and three mice were imaged from each stage. Branching was visible in both microdissected (arrow, A) and H&E-stained (arrow, C) P5 sections from controls but not in mutants (B and D). Ex vivo ultrasound imaging allowed visualization of the interior of anterior prostate of controls (G) that appears dense and white due to extensive epithelial infolding in the ducts, and the single layer of while epithelial lumen that outlines the main ducts of mutant mice (H). Arrowhead (in H) points to the significantly enhanced luminal space present within the main duct of mutant anterior prostate. H&E staining of prostates obtained from 6-month-old (I and K) contol and (J and L) mutant mice. Note that the widest region at the base of control prostate (K) fits into the field of view, whereas the narrowest region of mutant prostate (L) does not fit into the field of view. Epithelial infolding that spans the lumen was more extensive in control prostates (arrow, I) than mutant prostates (arrow, J). Insets represent higher magnification of the regions marked by the dotted square in K and L, respectively, that revealed nuclei with normal characteristics. Immunofluorescent staining of Klf6 expression in prostate tissues obtained from 8-week-old control (M and O) and mutant (N and P) mice. Klf6, which is a transcription factor, was highly localized to the nuclei of control prostate epithelium (arrows, O), and depletion of Klf6 in the mutant prostate was confirmed by the absence of green signal in the nuclei (arrowhead, P). I, J, M, and N, low magnification and high magnification (K, L, O, and P) of respective images. Bar in A, 500 um for A and B, 1000 um for E and F, and 750 um for G and H. Bar in C, 50 um for C and D. Bar in K, 200 um for I and J and 100 um for K-N. Bar in O, 50 um for O and P.

Copyright

This image is from Leow CC, J Biol Chem 2009 Jul 31;284(31):21057-65 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:153183

Associated Alleles

Symbol	Name
Klf6^tm1Fjs	Kruppel-like transcription factor 6; targeted mutation 1, Frederic J de Sauvage
Nkx3-1^tm3(cre)Mms	NK3 homeobox 1; targeted mutation 3, Michael M Shen

Associated Genotypes

Allelic Composition	Genetic Background
Klf6^tm1Fjs/Klf6^tm1Fjs Nkx3-1^tm3(cre)Mms/Nkx3-1^+	involves: 129/Sv * C57BL/6