Phenotype Image Detail

Image

Caption

Altered dopamine neurons in the ventral midbrain of Pten^tm1Hwu/Pten^tm1Hwu Slc6a3^{tm1.1(cre)Bkmn}/Slc6a3⁺ (KO) mice. Sections processed for tyrosine hydroxylase (TH) immunostaining show a significant increase in the number of TH immunopositive profiles in the substantia nigra compacta (SNc) and ventral tegmental area (VTA) of adult mutant animals when compared to controls (A and B). The total area covered by TH positive staining was significantly increased in mutant animals when compared to controls. Increase in the size of the mesolimbic and nigrostriatal projecting structures are due not only to an increase in the number of dopamine (DA) neurons, but also to an increase in neuronal size and in dendritic outgrowth (see inserts in A and B). Sections correspond to Bregma -3.16 mm with reference to Franklin and Paxinos (1997). Scale bar corresponding to high magnification, 250 um, and low magnification, 25 um.

Copyright

This image is from Diaz-Ruiz O, PLoS One 2009;4(9):e7027, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:153622

Associated Alleles

Symbol	Name
Pten^tm1Hwu	phosphatase and tensin homolog; targeted mutation 1, Hong Wu
Slc6a3^tm1.1(cre)Bkmn	solute carrier family 6 (neurotransmitter transporter, dopamine), member 3; targeted mutation 1.1, Cristina M Backman

Associated Genotypes

Allelic Composition	Genetic Background
Pten^tm1Hwu/Pten^tm1Hwu Slc6a3^tm1.1(cre)Bkmn/Slc6a3^+	involves: 129S4/SvJae * C57BL/6