Phenotype Image Detail

Image

Caption

Del(15Krt7-Krt18)1Tmm/Del(15Krt7-Krt18)1Tmm (KO) embryos show reduced and mislocalized desmoplakin, whereas adherens junctions and cell polarity are unaltered. (A and B) Double immunolabeling of keratin and desmoplakin (DP) on yolk sac indicates altered distribution and size of desmosomes in the presence (A) and absence (B) of keratins in yolk sac tissue. (C) Ninein part of the microtubule-organizing center complex at the centrosomes is located apically in wild-type (WT) (C) and mutant embryonic intestine (C'). (D) Note the apical localization of occludin, a transmembrane protein of tight junctions. (E) Antibody staining of the tight junction marker ZO-1 in the yolk sac revealed no changes in WT (E) compared with the mutant (E') E9.5 embryos. (D and E) ZO-1 and occludin are located at the apical plasma membrane of the yolk sac and the intestine, respectively, indicating normal cell polarity. (F) Intact adherens junctions in mutants (F') and their WT littermates (F), as demonstrated by staining for E-cadherin (E-cad) in yolk sac tissues of E9.5 embryos. lu, lumen. Bars: (A-B" and E-F') 10 um; (C-D') 2 um.

Copyright

This image is from Vijayaraj P, J Cell Biol 2009 Oct 19;187(2):175-84, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:153804

Associated Alleles

Symbol	Name
Del(15Krt7-Krt18)1Tmm	deletion, Chr 15, Thomas M Magin 1

Associated Genotypes

Allelic Composition	Genetic Background
Del(15Krt7-Krt18)1Tmm/Del(15Krt7-Krt18)1Tmm	involves: 129S7/SvEvBrd * C57BL/6