Phenotype Image Detail

Image

Caption

Neural crest and cranial nerve abnormalities in Chd7^Gt(XK403)Byg/Chd7⁺ (Chd7^+/xk) embryos. (A-D) In situ hybridization with a Crabp1 probe, a marker of neural crest cells, at E10.75. White arrows denote 2 streams of neural crest cells populating the fourth and sixth pharyngeal arches. Red arrows show a population of cells in the region of the foregut. (A and C) Wild-type (WT) Crabp1 expression at E10.5 in the pharyngeal region. (B and D) Right and left lateral view of heterozygous mutant embryos. The caudal stream of neural crest cells was reduced or absent. (E-H) Immunohistochemistry to detect neurofilaments at E10.5, with complete staining of cranial nerves V (trigeminal), VII (facial), VIII (vestibulocochlear), IX (glossopharyngeal), X (vagus), and XI (accessory). (E and G) In WT embryos, the trigeminal nerve was properly condensed and made full projections into the first branchial arch. (F and H) Heterozygous mutant embryos showed reduced staining of all the nerves (expected locations indicated by arrows). The trigeminal nerve was not condensed, and the projections into the branchial arches were not complete. The vagus nerve was reduced and sometimes fused with the glossopharyngeal nerve. Scale bars: 200 um.

Copyright

This image is from Randall V, J Clin Invest 2009 Nov;119(11):3301-10 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:154590

Associated Alleles

Symbol	Name
Chd7^Gt(XK403)Byg	chromodomain helicase DNA binding protein 7; gene trap XK403, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Chd7^Gt(XK403)Byg/Chd7^+	involves: 129P2/OlaHsd * C57BL/6