Phenotype Image Detail

Image

Caption

Histological characterization of 3-week-old tibial growth plates in wild-type (wt/wt), heterozygous Col10a1^tm1.1Rpbh/Col10a1⁺ (wt/m) and homozygous Col10a1^tm1.1Rpbh/Col10a1^tm1.1Rpbh (m/m) mice. Collagen X immunohistochemistry for metaphyseal chondrodysplasia type Schmid (MCDS) and control mice. Purple staining indicates collagen X localisation; The black boxed photomicrographs represent an expanded view of the indicated areas in the sections from 3 week old mice. The intracellular accumulation of collagen X in the homozygous mutant growth plates is apparent in the upper hypertrophic zone. The transition region in which intracellular accumulation of collagen X resolves accompanied by a limited secretion of mutant collagen X is indicated by the white horizontal arrows in the expanded view of the 3 week homozygous mutant sample. (B) In situ hybridisation for Col10a1, BiP, Col2a1 (collagen II) and Opn (osteopontin) in tibial growth plate sections from 3 week old animals. The presence of the transcript is indicated by blue staining. The hypertrophic zone is indicated by the vertical red brackets.

Copyright

This image is from Rajpar MH, PLoS Genet 2009 Oct;5(10):e1000691, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:154052

Associated Alleles

Symbol	Name
Col10a1^tm1.1Rpbh	collagen, type X, alpha 1; targeted mutation 1.1, Raymond P Boot-Handford

Associated Genotypes

Allelic Composition	Genetic Background
Col10a1^tm1.1Rpbh/Col10a1^tm1.1Rpbh	involves: 129S1/Sv * 129X1/SvJ * C57BL/6