Phenotype Image Detail

Image

Caption

Characterization of the Tg(Col10a1-Tg*L2263P)1Rpbh/0 (+/c) and Tg(Col10a1-Tg*L2263P)1Rpbh/Tg(Col10a1-Tg*L2263P)1Rpbh (c/c) mouse phenotype. (A) Characterisation of the tibial hypertrophic zone expansion in new born, 3 week and 6 week old mice: (i) H & E staining at the different time points (vertical red brackets indicates the extent of the hypertrophic zone, horizontal white scale bar = 100 um); (ii) Measurement of hypertrophic zone widths in the different genotypes (mean +/- SEM (n), *p < 0.05 for c/c vs +/+, **p < 0.001 for pooled c/c and +/c vs +/+. (B) Collagen X immunohistochemistry in new born and 3 week old mice (vertical red brackets indicates the extent of the hypertrophic zone, horizontal white scale bar = 100 um). (C) TRAP staining for osteoclasts at the vascular invasion front (vif - arrowheads, hz = hypertrophic zone): (i) Vertical arrows indicate positively stained cells (red/brown colour); (ii) Histogram depicting number of positive cells per 1 mm of vascular invasion front. *p < 0.05, **p < 0.01 (n = 3 for each genotype). (D) X-ray images of 6 week old mice, scale bar = 1 cm. (E) Femoral length measurements (mean + SEM (n): *p < 0.05 vs c/c; **p < 0.01 vs +/+; {p < 0.05 vs pooled c/+ and c/c mutant).

Copyright

This image is from Rajpar MH, PLoS Genet 2009 Oct;5(10):e1000691, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:154052

Associated Alleles

Symbol	Name
Tg(Col10a1-Tg*L2263P)1Rpbh	transgene insertion 1, Raymond P Boot-Handford

Associated Genotypes

Allelic Composition	Genetic Background
Tg(Col10a1-Tg*L2263P)1Rpbh/Tg(Col10a1-Tg*L2263P)1Rpbh	Not Specified
Tg(Col10a1-Tg*L2263P)1Rpbh/0	Not Specified