Phenotype Image Detail

Image

Caption

Decreased thymic cellularity and impaired thymocyte viability in Lrrc8a^tm1.1Geha/Lrrc8a^tm1.1Geha (Lrrc8a-/-) mice. (A, C, D, E) Gross appearance (A), H&E staining (C; bars: (left) 500 um; (right) 30 um; C = cortex, M = medulla; red asterisks in the inset indicate pyknotic nuclei), TUNEL staining (D, bars = 100 um) with quantitative analysis of TUNEL+ nuclei and immunostaining of thymic sections using a rabbit antibody that recognizes the p17 subunit, but not the precursor form, of caspase 3 (E, left; bars = 100 um) or the rabbit anti-caspase 3 antibody combined with anti-CD3 (E, right; bars = 20 um; caspase 3, blue and CD3, brown) with quantification of the results of thymi from the mutant mice and wild-type (WT) littermates (E).

Copyright

This image is from Kumar L, J Exp Med 2014 May 5;211(5):929-42, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:211308

Associated Alleles

Symbol	Name
Lrrc8a^tm1.1Geha	leucine rich repeat containing 8A VRAC subunit A; targeted mutation 1.1, Raif S Geha

Associated Genotypes

Allelic Composition	Genetic Background
Lrrc8a^tm1.1Geha/Lrrc8a^tm1.1Geha	B6.129S1(Cg)-Lrrc8a^tm1.1Geha