Phenotype Image Detail

Image

Caption

Lymphangiogenesis and severity of herpes simplex virus (HSV) keratitis and suture-induced inflammatory lymphangiogenesis is reduced in Lgals8^{tm1(KOMP)Vlcg}/Lgals8^{tm1(KOMP)Vlcg} (Gal-8 KO) mice. Wild-type (WT) (N = 13) and mutant (N = 8) mouse corneas were scarified and infected with 1 X 10⁶ pfu of HSV-1. The progression of HSV keratitis was assessed by corneal opacity scores on day 3, 6 and 8 post infection (c). Representative photomicrographs of eyes on day 8 post infection are shown in the right panel. After 8 day post infection, the corneal flat mounts were stained with anti-LYVE-1 and lymphatic vessel areas were quantified (d). Representative fluorescence images are shown in the right panel. (e) Suture-induced inflammatory lymphangiogenesis is reduced in mutant mice. Sutures were placed 2 mm above the limbal vessel in the corneas of WT (N = 9) and mutant (N = 9) mice. After 7 day post-surgery, the corneal flat mounts were stained with anti-LYVE-1 and lymphatic vessel areas were quantified. Representative fluorescence images are shown in the right panel. Data are plotted as mean s.e.m. and analysed using Student's t-test. *P < 0.05, **P < 0.01, ***P < 0.001 versus PBS (b) or WT (c,d,e). Scale bars: 800 um (b,d); 200 um (e).

Copyright

This image is from Chen WS, Nat Commun 2016;7():11302, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:235778

Associated Alleles

Symbol	Name
Lgals8^tm1(KOMP)Vlcg	lectin, galactose binding, soluble 8; targeted mutation 1, Velocigene
Lgals8^tm1(KOMP)Vlcg	lectin, galactose binding, soluble 8; targeted mutation 1, Velocigene

Associated Genotypes

Allelic Composition	Genetic Background
Lgals8^tm1(KOMP)Vlcg/Lgals8^tm1(KOMP)Vlcg	involves: C57BL/6NTac