Phenotype Image Detail

Image

Caption

Cell proliferation of the palatal shelf is reduced in Sox11^tm1.1Gan/Sox11^tm1.1Gan Tg(EIIa-cre)C5379Lmgd/0 (Sox11^Ella-Cre) mice. A and B, immunohistochemistry shows the BrdU incorporation in E13.5 palatal shelves. In comparison with the wild-type (Wt, A), the BrdU-labeled cells in the mutant were significantly reduced in both the epithelium and the mesenchyme (B). A', A", B', and B", large magnification of the boxed regions represent the distal palatal region (A' and B') and bend region (A" and B") in A and B, respectively. Scale bars = 100 um. C, statistical data show the relative proliferation folds in the distal palatal shelf of the Wt (A') and mutant (B'). D, statistical analysis shows the relative proliferation folds in the bend region in Wt control (A", n = 7) and mutant mice (B", n = 7). E and F, immunostaining shows a decrease in cyclin D1 expression in the proximal region of the palatal shelf in the mutant (F, arrowhead) in comparison with the Wt (E, arrowhead). E' and F' are enlarged pictures of the boxed areas in E and F, respectively. G and H, in situ hybridization exhibits reduced expression of cyclin D2 in the proximal region of the palatal shelf in mutant mice (F, arrowhead) compared with the Wt mice (G, arrowhead). T, tongue; Ps, palatal shelf.

Copyright

This image is from Huang H, J Biol Chem 2016 Mar 25;291(13):7107-18 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:232434

Associated Alleles

Symbol	Name
Sox11^tm1.1Gan	SRY (sex determining region Y)-box 11; targeted mutation 1.1, Lin Gan
Tg(EIIa-cre)C5379Lmgd	transgene insertion C5379, Laboratory of Mammalian Genes and Development, Heiner Westphal

Associated Genotypes

Allelic Composition	Genetic Background
Sox11^tm1.1Gan/Sox11^tm1.1Gan Tg(EIIa-cre)C5379Lmgd/0	involves: 129S6/SvEvTac * C57BL/6 * FVB/N