Phenotype Image Detail

Image

Caption

Role of Elavl1 (HuR) in intestinal integrity and progenitor cell survival. (A) Abnormal appearance of the GI tract 4 days after deletion of Elavl1 in Elavl1^tm1Thla/Elavl1^tm1Thla Gt(ROSA)26Sor^{tm1(cre/ERT2)Alj}/Gt(ROSA)26Sor⁺ mice.. Note the distended stomach and thin-walled intestinal tract devoid of food. (B) H&E stain of intestinal tissue sections from Elavl1^tm1Thla/Elavl1^tm1Thla (Elavl1^f/f) and Elavl1^tm1Thla/Elavl1^tm1Thla Gt(ROSA)26Sor^{tm1(cre/ERT2)Alj}/Gt(ROSA)26Sor⁺ (Elavl1^delta/delta) mice 4 days after tamoxifen administration. Note the effect of Elavl1 deletion on villus atrophy, epithelial cell disruption in the small intestine, and loss of tissue architecture and goblet cells in the colon. D, duodenum. Original magnification, 20. Scale bar: 50 um. (C) Immunohistochemical staining of small intestinal tissue sections from Elavl1^f/f and Elavl1^delta/delta mice 48 hours after tamoxifen treatment with either Elavl1 or Ki67 antibody or TUNEL stain. Note that although Elavl1 is primarily expressed in the epithelial cells, some stromal cells in the lamina propria also show immunoreactivity. Original magnification, 20. Scale bar: 50 um. (D) Immunohistochemical staining of colon sections from Elavl1^f/f and Elavl1^delta/delta mice 48 hours after tamoxifen treatment with either H&E stain, TUNEL, or Ki67 stain. Original magnification, 400 (first and third rows); 600 (second and fourth rows). (E) IB analysis of E-cadherin and beta-catenin expression in intestinal tissue extracts isolated from Elavl1^delta/delta mice upon tamoxifen treatment. (F) Immunohistochemical staining of F4/80 (myeloid marker) in small intestinal tissue sections from Elavl1^f/f and Elavl1^delta/delta mice 48 hours after tamoxifen treatment. Note that an intact villus was chosen to highlight the lamina propria and the epithelial compartments. Original magnification, 20. Scale bar: 50 um.

Copyright

This image is from Ghosh M, J Clin Invest 2009 Dec;119(12):3530-43 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:155109

Associated Alleles

Symbol	Name
Elavl1^tm1Thla	ELAV like RNA binding protein 1; targeted mutation 1, Timothy Hla
Gt(ROSA)26Sor^tm1(cre/ERT2)Alj	gene trap ROSA 26, Philippe Soriano; targeted mutation 1, Alexandra L Joyner

Associated Genotypes

Allelic Composition	Genetic Background
Elavl1^tm1Thla/Elavl1^tm1Thla Gt(ROSA)26Sor^tm1(cre/ERT2)Alj/Gt(ROSA)26Sor^+	involves: 129