Phenotype Image Detail

Image

Caption

Hyperplasia and hypertrophy of Pten^tm1Hwu/Pten^tm1Hwu Tg(Tyr-cre)1Lru/0 (HM) enteric neuronal cells (ENCs). (A) Transverse sections of wild-type (WT) and HM colons at P15: H&E staining, immunostaining with antibodies directed against TUJ1, NF, and Syn. Note that HM ganglia were bigger than WT ganglia. Scale bar: 50 um. (B) Transverse sections of WT, heterozygous (HT), and HM colons at P15. The surface of enteric nervous system (ENS) cells was estimated using ImageJ software. Note that HM ENS cells are twice as big as WT and HT ENS cells. ***P < 0.001. Scale bar: 25 um. (C) Transverse sections of WT, HT, and HM small intestine and colon in the proximal, middle, and distal parts immunostained with TUJ1 antibody and counter-stained with DAPI. The number of DAPI+ and TUJ1+ cells was determined in each compartment and plotted. Note that the number of DAPI+ and TUJ1+ cells is higher in the colon than in the small intestine. Scale bar: 25 um. Dotted lines enclose ENS. Error bars represent mean + SEM.

Copyright

This image is from Puig I, J Clin Invest 2009 Dec;119(12):3586-96 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:155110

Associated Alleles

Symbol	Name
Pten^tm1Hwu	phosphatase and tensin homolog; targeted mutation 1, Hong Wu
Tg(Tyr-cre)1Lru	transgene insertion 1, Lionel Larue

Associated Genotypes

Allelic Composition	Genetic Background
Pten^tm1Hwu/Pten^tm1Hwu Tg(Tyr-cre)1Lru/0	B6.Cg-Pten^tm1Hwu Tg(Tyr-cre)1Lru