Phenotype Image Detail

Image

Caption

Ate1^tm2.1Akas/Ate1^tm2.1Akas Tg(Tek-cre)12Flv/0 (CKO) mice have delayed development and die at early post-implantation stages. (A) Uterus excised from pregnant CKO female mated to CKO male at 12.5 day post coitum (12.5 dpc); arrowheads indicate the embryos that have implanted but either died or were severely delayed in development compared to their littermates. (B) Individual embryos excised from E8.5 uterus derived from CKO X CKO mating. Left panel shows the embryos that appeared normal in size and morphology. Right panel shows the embryos from the same uterus that appeared dead and partially deteriorated at E8.5. (C) Cross section through the uteri derived from CKO females mated to CKO males, showing individual littermate embryos at E7.5 (top) and E8.5 (bottom). While approximately half of the embryos at each stage appeared normal (left panels), the other half of the embryos in the same uterus were severely deteriorated (right panels), suggesting that these embryos have been dead by the time of dissection. (D) Left, examples of individual embryos at 4-8 cell stage (top), morula (middle), and blastocyst (bottom), derived from pregnant females at E2.5 and cultured for 1 day in vitro. Percentages in the columns on the right are calculated from the numbers of embryos found as 4-8 cell, morula, and blastocyst stage as marked on the left, derived from Ate1-floxed (WT) and CKO matings.

Copyright

This image is from Leu NA, PLoS One 2009;4(11):e7734, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:155427

Associated Alleles

Symbol	Name
Ate1^tm2.1Akas	arginyltransferase 1; targeted mutation 2.1, Anna Kashina
Tg(Tek-cre)12Flv	transgene insertion 12, Richard A Flavell

Associated Genotypes

Allelic Composition	Genetic Background
Ate1^tm2.1Akas/Ate1^tm2.1Akas Tg(Tek-cre)12Flv/0	involves: 129S4/SvJaeSor * 129S6/SvEvTac * C3H * C57BL/6 * CD-1