Phenotype Image Detail

Image

Caption

Increased genomic instability and apoptosis in the central nervous system of Paxx^em1Spj/Paxx^em1Spj Nhej1^tm1Fwa/Nhej1^tm1Fwa (Paxx-/- Xlf-/-) embryos. Representative images depicting immunohistochemical staining for cleaved caspase 3-positive apoptotic cells (A) and gammaH2AX-positive cells (B) in the neural tubes of E14.5 embryos. Bar graphs (mean SD) present the percentage of cleaved caspase 3-positive (A) and pan-nuclear gammaH2AX-positive (B) cells of the representative genotypes. More than 500 cells per embryo were counted. n >/= 3 per genotype. Statistical analysis was performed using one-way ANOVA. (****) P < 0.0001, Dunnett's multiple comparisons test.

Copyright

This image is from Balmus G, Genes Dev 2016 Oct 1;30(19):2152-2157, and is displayed with the permission of Cold Spring Harbor Laboratory Press, who owns the Copyright. J:236776

Associated Alleles

Symbol	Name
Nhej1^tm1Fwa	non-homologous end joining factor 1; targeted mutation 1, Frederick W Alt
Paxx^em1Spj	non-homologous end joining factor; endonuclease-mediated mutation 1, Stephen P Jackson

Associated Genotypes

Allelic Composition	Genetic Background
Paxx^em1Spj/Paxx^em1Spj Nhej1^tm1Fwa/Nhej1^tm1Fwa	involves: 129S6/SvEvTac * C57BL/6NTac