Phenotype Image Detail

Image

Caption

Sox2 is required for goblet cell differentiation after pulmonary allergen sensitization. Lung sections from normal four week old mice were co-stained for mucins with Alcian blue and Sox2 demonstrating the presence of goblet cells in the airways of control mice (A) but not in Sox2^tm1.1Lan/Sox2^tm1.1Lan Tg(Scgb1a1-cre)1Kkw/0 (Sox2^delta/delta) littermates (B). Response to allergen challenge (C-H): In control mice, Alcian blue reactive cells expressed Sox2 (C,D), and the goblet cell associated proteins Spdef (E,F) and Muc5AC (G,H). Extensive goblet cell differentiation was observed in bronchi and larger bronchioles of control (C,E,G) but not mutant mice (D,F,H), in spite of the robust inflammatory response seen in both groups. Asterisk in D indicates a small cluster of Sox2-positive (untargeted) goblet cells. Arrows indicate regions shown in insets. Scale bar is 50 um, inset scale bar is 10 um. OVA+, ovalbumin treatment.

Copyright

This image is from Tompkins DH, PLoS One 2009;4(12):e8248, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:155963

Associated Alleles

Symbol	Name
Sox2^tm1.1Lan	SRY (sex determining region Y)-box 2; targeted mutation 1.1, Richard A Lang

Associated Genotypes

Allelic Composition	Genetic Background
Sox2^tm1.1Lan/Sox2^tm1.1Lan Tg(Scgb1a1-cre)1Kkw/0	involves: 129/Sv * C57BL/6