Phenotype Image Detail

Image

Caption

Formation of large lysosomes is impaired in the yolk sac visceral endoderm (VE) cells of Enpp2^tm1Mmas/Enpp2^tm1Mmas (Enpp2-/-) embryos. A, IgG immunostaining (top), electron microscopy (middle), and LysoTracker Red staining (bottom) of the E8.5 yolk sac. Immunostaining with anti-mouse IgG antibody detects endocytic vesicles in the VE cells (ve). IgG-containing vesicles in Enpp2^tm1Mmas/Enpp2^tm1Mmas VE cells were smaller than those in the wild-type (WT) cells. Electron microscopy showed a few large vesicles in the apical portion of the WT VE cells (arrows), whereas numerous vesicles of smaller size were seen in the mutant cells (arrows). Confocal microscopic images of the yolk sac showed that LysoTracker Red positive lysosomes were decreased in size but increased in number in the mutant VE cells compared with those in the control. The yellow dotted lines indicate the boundary of VE cells. Scale bar represents 20 um (top), 1.7 um (middle), and 12 um (bottom). Abbreviations used are as follows: bc, blood cells; mv, microvillus; n, nucleus. B, size and number of lysosomes in VE cells. Lysosomes were significantly decreased in size and increased in number in mutant VE cells. The values are the means +/- S.E. (***, p<0.001; unpaired t test). The number of embryos examined is shown in each column.

Copyright

This image is from Koike S, J Biol Chem 2009 Nov 27;284(48):33561-70 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:156013

Associated Alleles

Symbol	Name
Enpp2^tm1Mmas	ectonucleotide pyrophosphatase/phosphodiesterase 2; targeted mutation 1, Masayuki Masu

Associated Genotypes

Allelic Composition	Genetic Background
Enpp2^tm1Mmas/Enpp2^tm1Mmas	involves: 129P2/OlaHsd * C57BL/6N