Phenotype Image Detail

Image

Caption

Abnormal lymphatic vessel maturation in Lyve1^{tm1.1(EGFP/cre)Cys}/Lyve1⁺ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh mice. (A) Confocal images showing lymphatic vessels stained with antibody against LYVE-1 in whole mount of mouse trachea. Arrowheads point to the jagged appearance of the lymphatic vessels in Lyve1^{tm1.1(EGFP/cre)Cys}/Lyve1⁺ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh (Sphk delta) mice. (B) Confocal images showing the button-like pattern of VE-cadherin at endothelial cell-cell junctions of the diaphragm initial lymphatics. Arrowheads mark VE-cadherin+ buttons in the control and a corresponding junctional region in the mutant mice. White squares (top) indicate enlarged regions (bottom). Data are representative of six experiments (n = 6 mice). (C) VE-cadherin buttons per 1,000-um² projected area of lymphatic for littermate control and mutant trachea (n = 3 mice per group). Bars show means SE. *, P < 0.05 (Student's t test).

Copyright

This image is from Pham TH, J Exp Med 2010 Jan 18;207(1):17-27, S1-4, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:156826

Associated Alleles

Symbol	Name
Lyve1^tm1.1(EGFP/cre)Cys	lymphatic vessel endothelial hyaluronan receptor 1; targeted mutation 1, Jason G Cyster
Sphk1^tm2Cgh	sphingosine kinase 1; targeted mutation 2, Shaun R Coughlin
Sphk2^tm1.1Cgh	sphingosine kinase 2; targeted mutation 1.1, Shaun R Coughlin

Associated Genotypes

Allelic Composition	Genetic Background
Lyve1^tm1.1(EGFP/cre)Cys/Lyve1^+ Sphk1^tm2Cgh/Sphk1^tm2Cgh Sphk2^tm1.1Cgh/Sphk2^tm1.1Cgh	involves: 129P2/OlaHsd * 129S4/SvJae * C57BL/6