Phenotype Image Detail

Image

Caption

Excessive Wnt/beta-catenin signaling results in anterior head defects during embryonic development. (A) Whole mounts of E9.5 wild-type (WT, +/+) and Apc^Min/Apc^tm1Tno (min/fl) mutant embryos and E17.5 Apc⁺/Apc^tm1Tno(+/fl) and Apc^Min/Apc^tm1Tno (min/fl) mutant embryos. (B) Whole mounts of E13.5 wild-type and mutant embryos of the indicated genotypes. Genetic ablation of one allele of beta-catenin in (min/fl) headless mutant (Apc^Min/Apc^tm1Tno Ctnnb1^tm4.1Wbm/Ctnnb1⁺) rescues normal head morphology in (min/fl; +/-) mice. (C) Histological cross sections of E12 wild-type (+/+) and mutant (min/fl) embryos. (D) Confocal cross section of E3.5-5.5 (+/fl) and (min/fl) embryos stained for beta-catenin protein. The arrowheads demarcate the outer layer (anterior visceral endoderm) of the embryo which shows increased and expanded beta-catenin expression in the (min/fl) mutant. A, Anterior; P, Posterior. (E) Whole mount in vivo X-gal staining of E16 embryos to monitor canonical Wnt/beta-catenin dependent activity in compound mutant mice harboring the corresponding BAT::gal reporter transgene. Genotypes are as follows: wild-type (+/+); Apc^+/fl (+/fl); Apc^fl/fl (fl/fl); Apc^min/+ (min/+); Apc^min/fl (min/fl); Apc^min/fl;Ctnnb1^+/- (min/fl; +/-).

Copyright

This image is from Buchert M, PLoS Genet 2010 Jan;6(1):e1000816, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:156864

Associated Alleles

Symbol	Name
Apc^Min	APC, WNT signaling pathway regulator; multiple intestinal neoplasia
Apc^tm1Tno	APC, WNT signaling pathway regulator; targeted mutation 1, Tetsuo Noda
Ctnnb1^tm4.1Wbm	catenin beta 1; targeted mutation 4.1, Walter Birchmeier

Associated Genotypes

Allelic Composition	Genetic Background
Apc^Min/Apc^tm1Tno	involves: 129 * 129S4/SvJae * C57BL/6
Apc^Min/Apc^tm1Tno Ctnnb1^tm4.1Wbm/Ctnnb1^+	involves: 129 * 129P2/OlaHsd * 129S4/SvJae * C57BL/6