Phenotype Image Detail

Image

Caption

Analysis of lymphatic structure and function in Nrp2^tm1Ddg/Nrp2^tm1Ddg (nrp2+/-) and Nrp2^tm1Ddg/Nrp2⁺ Flt4^tm1Ali/Flt4⁺ (nrp2+/- vegfr3+/-) mice. (A-E) Whole-mount staining using an anti-VEGFR3 (FLT4) antibody to label lymphatic vessels in E13.5 mouse embryos (A and B) and P0 hearts (C-E) of the indicated genotypes. Note reduced vessel coverage in the homozygous Nrp2^tm1Ddg/Nrp2^tm1Ddg embryo heads (B, black arrows) compared with wild-type (WT) littermates (A, white arrows). e, eye. Also note abnormal morphology of lymphatic vessel sprouts at the surface of the heart in the homozygous mutant (E, black arrows) compared with WT and heterozygous littermates (C and D, white arrows). (F and G) Xgal staining of hearts from 2-month-old heterozygous Flt4^tm1Ali/Flt4⁺ (vegfr3+/-) (F) and double-heterozygous Flt4^tm1Ali/Flt4⁺ (nrp2+/- vegfr3+/-) (G) mice. (H and I) X-gal staining of diaphragm. Note reduced lymph vessel branching in the double-heterozygous tissues. (J and K) Transport of intradermally injected FITC dextran in ears of mice of the indicated genotypes. Asterisks indicate the injection site. Note that double-heterozygous lymph vessels (K) are poorly branched when compared with heterozygous (J) but transport the dextran normally. WT, wild type. (L-O) Bright-field images of VEGFR3-stained lymph vasculature in the dorsal trunk skin at P0. Note enlarged, poorly branched vessels in the double heterozygous (N) compared with WT (L). Numerous lymphatic tips are present in WT (L) but only few in double heterozygotes (N). (M and O) Higher magnifications of boxed regions in L and N, respectively, illustrating morphology of lymphatic tips. Note filopodial extensions of thin-tapered tips in WT (M) compared with enlarged, blunted tips in double heterozygotes (O). Bars: (A and B) 233 um; (C-E) 0.55 mm; (F and G) 0.8 mm; (H and I) 0.5 mm; (J and K) 1.6 mm; (L and N) 200 um; (M and O) 40 um.

Copyright

This image is from Xu Y, J Cell Biol 2010 Jan 11;188(1):115-30, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:156869

Associated Alleles

Symbol	Name
Flt4^tm1Ali	FMS-like tyrosine kinase 4; targeted mutation 1, Kari Alitalo
Nrp2^tm1Ddg	neuropilin 2; targeted mutation 1, David D Ginty

Associated Genotypes

Allelic Composition	Genetic Background
Flt4^tm1Ali/Flt4^+ Nrp2^tm1Ddg/Nrp2^+	involves: C57BL/6
Nrp2^tm1Ddg/Nrp2^tm1Ddg	involves: C57BL/6