Phenotype Image Detail

Image

Caption

Speedy A is required for telomere attachment to the nuclear envelope (NE) and for telomere cap exchange. Structurally preserved PD18 spermatocytes were used to analyze telomere attachment to the NE. Telomeres were stained with TRF1. (A-D) In wild-type (WT, Spdya+/+) spermatocytes, all telomeres were on the NE (arrows), whereas in Spdya^tm1.1Klad/Spdya^tm1.1Klad spermatocytes, telomeres were observed inside the nucleus (arrowheads). (E and F) Structurally preserved 17.5-dpc oocytes were used for analyzing telomere attachment to the NE. In WT zygotene oocytes, all telomeres were on the NE (arrows). However, in mutant zygotene-like oocytes, telomeres were inside the nucleus (arrowheads). (G-J) Superresolution microscopy images of PD18 spermatocytes showing the telomere cap exchange. Telomeres were stained with TRF2. (G and H) A representative WT pachytene spermatocyte showing the shelterin ring structure (arrows and Inset). (magnification: H and J, 10x G and I) (I and J) A representative mutant zygotene-like spermatocyte showing the absence of the shelterin ring structure (arrowheads).

Copyright

This image is from Tu Z, Proc Natl Acad Sci U S A 2017 Jan 17;114(3):592-597. Copyright 2017 National Academy of Sciences, U.S.A. J:239544

Associated Alleles

Symbol	Name
Spdya^tm1.1Klad	speedy/RINGO cell cycle regulator family, member A; targeted mutation 1.1, Phillip Kladis

Associated Genotypes

Allelic Composition	Genetic Background
Spdya^tm1.1Klad/Spdya^tm1.1Klad	involves: 129S1/Sv * C57BL/6J