Phenotype Image Detail

Image

Caption

Mirc32^tm1Thbr/Mirc32^tm1Thbr (miR-143/145 KO) mice develop neointimal lesions. (A-C) Sections of wild-type (WT) (A) and KO (B and C) femoral arteries are shown (scale bar, 100 um); boxed regions are magnified in D-F (scale bar, 30 um). Electron micrographs of early and late lesions are shown in G and H, respectively. Scale bars: 5 um (G), 2 um (H, inset). (I-K) Femoral artery of WT (I), Mirc32^tm1Thbr/Mirc32⁺ heterozygous (J), and Mirc32^tm1Thbr/Mirc32^tm1Thbr homozygous animals (K) stained for beta-galactosidase activity to indicate miR-143/145 locus activity (scale bar, 25 um). (L and M) Sections of WT (L) and KO vessels (M). Sections of the femoral artery stained with antibodies against alpha-SMA or the macrophage marker F8/40. (N) Sections of the femoral artery stained with antibodies against alpha-SMA and collagen I. Scale bar: 20 um (L-N). Lesions were frequently found in KO animals but not in WT animals. (G) Formation of neointima with SMCs and macrophages (not shown) between the lamina elastica and endothelial cells. Lesions at later stages reached large volumes and showed a thinned media (I-N, arrows) but no constriction of the vessel. Note the absence of foam cells or lipophilic inclusions (G and H), which was also indicated by the absence of Sudan IV staining (not shown). SMCs were recognized by morphology (G and H), by anti-smooth muscle staining (M and N), or by lacZ transgene expression under the control of the miR-143/145 locus (K). The plaques contain macrophages identified by the F4/80 marker (M) and deposits of amorphous collagen (N). Smooth muscle (G) and macrophages were also identified in early lesions (not shown). EL, elastic lamina; NI neointima. Scale bars: 25 um (I-K); 20 um (L-N).

Copyright

This image is from Boettger T, J Clin Invest 2009 Sep;119(9):2634-47 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:152702

Associated Alleles

Symbol	Name
Mirc32^tm1Thbr	microRNA cluster 32, including Mir143 and Mir145a; targeted mutation 1, Thomas Braun

Associated Genotypes

Allelic Composition	Genetic Background
Mirc32^tm1Thbr/Mirc32^tm1Thbr	involves: 129/Sv * C57BL/6