Phenotype Image Detail

Image

Caption

Abnormal Z line organization in E14.5 Casz1^{Gt(CJ0565)Wtsi}/Casz1^{Gt(CJ0565)Wtsi} (Casz1^{betageo/betageo}) heart. A, cardiomyocytes isolated from wild-type (WT, Casz1+/+) and mutant E14.5 embryo hearts were cultured in vitro for 2 days and immunostained for alpha-actinin (red) and F-actin (green), whereas the nuclei were stained with DAPI (blue). Representative images show that clear Z lines were presented in the WT cardiomyocytes, but not in the mutant cardiomyocytes. C, more representative images show that clear Z lines are present in the WT cardiomyocytes. There are no clear Z lines in these mutant cardiomyocytes, and the alpha-actinin staining pattern is distinct compared with the WT cardiomyocytes.

Copyright

This image is from Liu Z, J Biol Chem 2014 Oct 24;289(43):29801-16 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:242127

Associated Alleles

Symbol	Name
Casz1^Gt(CJ0565)Wtsi	castor zinc finger 1; gene trap CJ0565, Wellcome Trust Sanger Institute

Associated Genotypes

Allelic Composition	Genetic Background
Casz1^Gt(CJ0565)Wtsi/Casz1^Gt(CJ0565)Wtsi	involves: 129P2/OlaHsd * C57BL/6