Phenotype Image Detail

Image

Caption

The spleen of Ednrb^tm1Nrd/Ednrb^tm1Nrd Gt(ROSA)26Sor^{tm14(CAG-tdTomato)Hze}/Gt(ROSA)26Sor⁺ H2az2^{Tg(Wnt1-cre)11Rth}/0 animals is small in size and has abnormal architecture. (A) Gross photographs of representative spleens isolated from heterozygotes and homozygous mutant animals demonstrating the size difference. (B) H&E staining of spleen cross-sections from heterozygotes and hmozygous animals. Mutant spleens demonstrate decreased red pulp (RP) and white pulp (WP). Scale bar = 600 um. (C) IHC of spleen cross-sections with B220 (green) and CD3 (blue). There are decreased B-cells in the germinal centers and a paucity of these cells within the marginal zone, located outside the ring of MOMA+ (red) staining. Scale bar = 200 um.

Copyright

This image is from Gosain A, PLoS One 2015;10(6):e0128822, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:233811

Associated Alleles

Symbol	Name
Ednrb^tm1Nrd	endothelin receptor type B; targeted mutation 1, Noah Druckenbrod
Gt(ROSA)26Sor^tm14(CAG-tdTomato)Hze	gene trap ROSA 26, Philippe Soriano; targeted mutation 14, Hongkui Zeng
H2az2^Tg(Wnt1-cre)11Rth	H2A.Z histone variant 2; transgene insertion 11, David H Rowitch