Phenotype Image Detail

Image

Caption

Mcm9 mutations cause germ-cell depletion and loss of spermatogonial stem cells. (C and D) H&E stained histological sections of testes from 12-week-old wild-type (WT) and Mcm9^Gt(XG743)Byg/Mcm9^Gt(XG743)Byg (XG/XG) mice. (magnification: 200X). Error bars indicate SD. Seminiferous tubules marked with an asterisk (*) are devoid of germ cells; those marked with a caret (^) exhibit meiotic arrest (Inset); and those indicated by arrows are depleted of spermatogonia but undergoing a final wave of spermatogenesis. Circled tubules are examples of normal spermatogenesis, (E and F) H&E-stained histological sections of ovaries from 24-wk-old mice. (G-L) Immunofluorescence of 1-dpp testes (G and H) and ovaries (J and K) (magnification: 200X). MVH (green) stains germ cells and DAPI stains nuclei (blue). (I and L) Germ-cell counts from MVH staining data at 1 dpp (n = 3). *Significant difference vs. WT. XG, Mcm9^Gt(XG743)Byg/Mcm9^Gt(XG743)Byg; p53, Trp53-/-; p21, Cdkn1a-/-. Error bars indicate SD.

Copyright

This image is from Hartford SA, Proc Natl Acad Sci U S A 2011 Oct 25;108(43):17702-7. Copyright 2011 National Academy of Sciences, U.S.A. J:177420

Associated Alleles

Symbol	Name
Mcm9^Gt(XG743)Byg	minichromosome maintenance 9 homologous recombination repair factor; gene trap XG743, BayGenomics

Associated Genotypes

Allelic Composition	Genetic Background
Mcm9^Gt(XG743)Byg/Mcm9^Gt(XG743)Byg	involves: 129P2/OlaHsd * C3HeB/FeJ