Phenotype Image Detail

Image

Caption

Delayed spreading and abnormal lamellipodia in Ptk2^tm1Heen/Ptk2^tm1Heen (FAKdelta/delta) fibroblasts and endothelial cells. Wild-type (FAK+/+) and mutant mouse embryonic fibroblasts (MEFs) or endothelial cells (EC) were plated on polylysine (Pl) or fibronectin (Fn) for 0.5-20 hours as indicated. Cells were stained for actin with rhodamine-conjugated phalloidin (A-D and F) and/or antibodies recognizing FAK (A, D, and E), cortactin (B and F), and vinculin (C and F) and revealed with fluorescein isothiocyanate-conjugated secondary antibody. EC cells (E and F) were identified by CD31 immunostaining. Lamellipodia were well developed in wild-type (arrows) but not mutant cells (A, B and D-F), which displayed narrow cortactin-positive protrusions (B and F). Focal adhesions were more numerous and larger in mutant than WT cells (C-F). Scale bars, 20 um.

Copyright

This image is from Corsi JM, J Biol Chem 2009 Dec 11;284(50):34769-76 and is displayed with the permission of the American Society for Biochemistry and Molecular Biology who owns the Copyright. Full text from JBC. J:157804

Associated Alleles

Symbol	Name
Ptk2^tm1Heen	PTK2 protein tyrosine kinase 2; targeted mutation 1, Herve Enslen

Associated Genotypes

Allelic Composition	Genetic Background
Ptk2^tm1Heen/Ptk2^tm1Heen	involves: 129/Sv * C57BL/6