Phenotype Image Detail

Image

Caption

Absence of major vascular defects in Slc20a1^tm1.2Lbek/Slc20a1^tm1.2Lbek (delta5/delta5) mice. (A-B) Yolk sac membranes from wild-type (+/+) and mutant embryos illustrate the tree-like architecture of the vasculature (white arrow). Despite that mutant yolk sac appears white, vessels are present but often devoid of red blood cells (black arrow). (C) Gross appearance of wild-type (left) and mutant (right) E12.5 embryos with intact yolk sac membranes. (D-E) Close examiniation of wild-type and mutant yolk sac shows empty vessels in the mutant yolk sac (F-G) H&E staining of yolk sac cross-sections from wild-type and mutant embryos illustrate that, although null yolk sacs present with many vessels (black arrow), they are almost devoid of red blood cells (white arrow). (H-I) Anti-PECAM-1 IHC (+Ab) of wild-type and mutant yolk sac cross-sections at E12.5 illustrating the presence of endothelial cells in both genotypes (black arrow). A negative control (2Ab) is shown to illustrate the specificity of the signal. (J-K) Anti-PECAM-1 IHC (+Ab) of wild-type and mutant liver cross-sections at E12.5 illustrating the presence of endothelial cells in both genotypes. A negative control (2Ab) is shown to illustrate the specificity of the signal. Bar, 100 um.

Copyright

This image is from Beck L, PLoS One 2010;5(2):e9148, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:157997

Associated Alleles

Symbol	Name
Slc20a1^tm1.2Lbek	solute carrier family 20, member 1; targeted mutation 1.2, Laurent Beck

Associated Genotypes

Allelic Composition	Genetic Background
Slc20a1^tm1.2Lbek/Slc20a1^tm1.2Lbek	involves: 129S2/SvPas * BALB/c * C57BL/6 * C57BL/6J