Phenotype Image Detail

Image

Caption

Apoptotic and proliferation defect in Slc20a1^tm1.2Lbek/Slc20a1^tm1.2Lbek (delta5/delta5) fetal livers. (A-B) H&E staining of E12.5 livers illustrating the presence of large sinuses in mutants. Higher magnification views (boxes) illustrate the abundance of pyknotic nuclei (arrow) in the mutant liver. (C-D) TUNEL analysis on sections from the same fetal livers shown in (A-B). Note the significant number of positively stained cells (green) in the mutant livers. Nuclei were stained with DAPI (blue). (E-F) TUNEL analysis on E11.5 fetal livers sections revealed that apoptotic signals were similar in wild-type and mutant livers. (G-J) Activated caspase 3 staining of E12.5 (G,H) and E11.5 (I,J) mutant liver sections. White arrows indicate examples of positively stained cells. (K-L) Ki67 staining shows that while overall cell density is reduced, proliferation is reduced but ongoing in mutant embryos (arrow). (M-N) Pulse BrDU labeling of E11.5 embryos. Positively labeled cells (arrow) are less numerous in the mutant. Bar, 100 um.

Copyright

This image is from Beck L, PLoS One 2010;5(2):e9148, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:157997

Associated Alleles

Symbol	Name
Slc20a1^tm1.2Lbek	solute carrier family 20, member 1; targeted mutation 1.2, Laurent Beck

Associated Genotypes

Allelic Composition	Genetic Background
Slc20a1^tm1.2Lbek/Slc20a1^tm1.2Lbek	involves: 129S2/SvPas * BALB/c * C57BL/6 * C57BL/6J