Phenotype Image Detail

Image

Caption

Phenotype of Slc20a1^tm1Lbek/Slc20a1^tm1.2Lbek (neo/delta5) compound heterozygotes. (A) Morphological appearance of Slc20a1^tm1Lbek/Slc20a1^tm1.2Lbek E14.5 embryos (right), as compared to wild-type (+/+) littermates (left). Note the severe anemia and slight decrease in size. (B-C) H&E staining of E14.5 sagital sections from wild-type and mutant livers. The mutant liver shows disorganized parenchyme, reduced cellularity and pyknotic nuclei (white arrow). (D-E) H&E staining of E12.5 sections from wild-type and mutant livers showing no difference between the two genotypes. Bar, 100 mm. (F-G) Close examination of yolk sac membranes from wild-type and mutant E14.5 embryos demonstrate the presence of a highly developed vasculature (black arrows) characterized by a decrease in red blood cell number. (H-I) May Grunwald/Giemsa staining of peripheral blood smears from E14.5 wild-type and mutant littermates. Note the similar morphology of red blood cells, but the higher percentage of nucleated erythrocytes (black arrows).

Copyright

This image is from Beck L, PLoS One 2010;5(2):e9148, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:157997

Associated Alleles

Symbol	Name
Slc20a1^tm1.2Lbek	solute carrier family 20, member 1; targeted mutation 1.2, Laurent Beck
Slc20a1^tm1Lbek	solute carrier family 20, member 1; targeted mutation 1, Laurent Beck

Associated Genotypes

Allelic Composition	Genetic Background
Slc20a1^tm1Lbek/Slc20a1^tm1.2Lbek	involves: 129S2/SvPas * BALB/c * C57BL/6 * C57BL/6J