Phenotype Image Detail

Image

Caption

Ptpn11^tm4.2Bgn/Ptpn11⁺ (LS/+) mice have pathological cardiac hypertrophy. (A) Representative hearts from 16-week-old wild-type (WT) and heterozygous mutant mice. (B) Transverse sections of WT and mutant hearts stained with H&E (original magnification, 100). (C) Heart weight to body weight (HW/BW) ratios of WT and mutant mice at indicated ages (n = 5-10 mice/group at each age measured). Data represent mean SEM; *P < 0.05, 2-tailed Student's t test. (D) Paraffin-embedded heart sections from 16-week-old WT and mutant mice stained with reticulin (original magnification, 400; scale bar: 100 um). The average area of WT cardiomyocytes is 2,038 98.5 um²/cardiomyocyte and that of mutant cardiomyocytes is 2,578 115.9 um²/cardiomyocyte (P < 0.001, 2-tailed Student's t test). Data represent mean SEM (200-500 cardiomyocytes/group). (E) Cardiomyocytes from 8- to 10-week-old WT and mutant hearts (original magnification, 200). Cardiomyocyte length for WT hearts is 108.4 2.6 um and for mutant hearts is 113.5 2.9 um; cardiomyocyte width for WT hearts is 18.9 0.8 um and for mutant hearts is 22.4 0.7 um (P < 0.05, 2-tailed Student's t test). Data represent mean SEM (200-500 cardiomyocytes; n = 4 mice each.). (F) H&E staining (original magnification, 400; white bar: 100 um) of paraffin-embedded longitudinal heart sections from 16-week-old WT and mutant mice. Note the myofiber disarray and increased inflammatory cells (indicated by white arrows and magnified in inset) in the interstitial spaces. (G) Scanning electron microscopy of cardiomyocytes (original magnification, 4,800) from 12-week-old mice. Double arrowhead: 1 um.

Copyright

This image is from Marin TM, J Clin Invest 2011 Mar 1;121(3):1026-43 and is displayed with the permission of the American Society for Clinical Investigation who owns the Copyright. J:172033

Associated Alleles

Symbol	Name
Ptpn11^tm4.2Bgn	protein tyrosine phosphatase, non-receptor type 11; targeted mutation 4.2, Benjamin G Neel

Associated Genotypes

Allelic Composition	Genetic Background
Ptpn11^tm4.2Bgn/Ptpn11^+	involves: 129S6/SvEvTac * C57BL/6J * FVB/N