Phenotype Image Detail

Image

Caption

Complete repair of DNA double-strand breaks (DSBs) and crossover formation is not observed in Syce3^tm1Rben/Syce3^tm1Rben (Syce3^-/-) oocytes. (A) Immunofluorescence analysis on mouse oocytes collected from 19.5 days post coitum (dpc) wild-type (WT) and mutant littermates. WT and mutant oocytes double stained for gammaH2AX (first row), RPA (second row) or MLH1 (third row) and SYCP3 are shown. Images were acquired using a confocal laser scanning microscope. Bars, 10 um. (B) Numbers of RPA foci (left) in pachytene (WT, n = 21; mutant, n = 27) and diplotene (WT, n = 15; mutant, n = 15) oocytes depicted in a box-and whisker plot. Box-and-whisker plot of MLH1 foci (right) counted in pachytene (WT, n = 23; mutant, n = 26) and diplotene (WT, n = 18; mutant, n = 20) oocytes. (C) Pachytene/diplotene ratio of oocytes collected from control (n = 77) or mutant (n = 88) 19.5 dpc littermates.

Copyright

This image is from Schramm S, PLoS Genet 2011 May;7(5):e1002088, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:172714

Associated Alleles

Symbol	Name
Syce3^tm1Rben	synaptonemal complex central element protein 3; targeted mutation 1, Ricardo Benavente

Associated Genotypes

Allelic Composition	Genetic Background
Syce3^tm1Rben/Syce3^tm1Rben	involves: 129S1/Sv * 129X1/SvJ * C57BL/6