Phenotype Image Detail

Image

Caption

Mia3^tm1Lex/Mia3^tm1Lex (Mia3^-/-) embryos are dwarfed, edemic, and fail to form a mineralized skeleton. (A and B) Mutant embryos display a shortening of the snout and limbs at 16.5 days post coitum (dpc). (C and D) Mutant embryos are significantly dwarfed at 18.5 dpc. (E and F) Alizarin red-positive skeletal mineralization initiates within the intramembranous bone of the jaw and endochondral bones of the clavicles of 13.5-dpc wild-type (WT) embryos (arrows) but is absent from the Alcian blue-positive cartilage anlagen of mutants. (G and H) Mineralization is advanced in nearly all axial and appendicular elements in 15.5-dpc control embryos but is profoundly delayed in the null skeleton. (I-L) Calcified matrix is apparent in the bones of 18.5-dpc knockout animals (I and J), but micro-CT (n = 3) maximum intensity projections highlight complete absence of the mature ossified skeleton (K and L). Bars, 1 mm.

Copyright

This image is from Wilson DG, J Cell Biol 2011 May 30;193(5):935-51, and is displayed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported License. J:172737

Associated Alleles

Symbol	Name
Mia3^tm1Lex	MIA SH3 domain ER export factor 3; targeted mutation 1, Lexicon Pharmaceuticals
Mia3^tm1Lex	MIA SH3 domain ER export factor 3; targeted mutation 1, Lexicon Pharmaceuticals

Associated Genotypes

Allelic Composition	Genetic Background
Mia3^tm1Lex/Mia3^tm1Lex	involves: 129S5/SvEvBrd * C57BL/6