Phenotype Image Detail

Image

Caption

Structural and protein localization defects in Ttc8^tm1Reed/Ttc8^tm1Reed olfactory epithelium (OE). Olfactory epithelium cryosections from heterozygous (BBS8+/-) or homozygous Ttc8^tm1Reed/Ttc8^tm1Reed (BBS8-/-) animals were immunostained and imaged by confocal microscopy (A: single plane, B and C: flattened Z-stacks). (A) Cilia abundance, visualized by acetylated tubulin, is dramatically reduced in homozygous mutant mice. (B) The signal transduction proteins ACIII and ORI7 show reduced intensity in the cilia layer and mislocalization within the dendrite in homozygous mutant OE. (C) SLP3 shows reduced signal at the cilia layer and accumulation within the dendrite and apical cell body. (D) Scanning electron microscopy (SEM) of the olfactory epithelium of 37-week-old littermates. [Scale bar: 10 um (A-C); 2 um (D).]

Copyright

This image is from Tadenev AL, Proc Natl Acad Sci U S A 2011 Jun 21;108(25):10320-5. Copyright 2011 National Academy of Sciences, U.S.A. J:173465

Associated Alleles

Symbol	Name
Ttc8^tm1Reed	tetratricopeptide repeat domain 8; targeted mutation 1, Randall R Reed

Associated Genotypes

Allelic Composition	Genetic Background
Ttc8^tm1Reed/Ttc8^tm1Reed	involves: 129