Phenotype Image Detail

Image

Caption

Retinal degeneration and renal anomalies in Ttc8^tm1Reed/Ttc8^tm1Reed (BBS8-/-) mice. (A) Retinal sections from adult heterozygous (BBS8+/-) and homozygous mutant mice were immunostained for rhodopsin and counterstained with DAPI. A dramatic thinning of the outer nuclear layer (ONL) is apparent in homozygous mutant tissue. (B) ONL thickness was measured from n=2 mice/genotype. Sections from various regions were used, and at least four measurements were taken per animal. The ONL is significantly thinner in the homozygous mutant (P<0.001). (C) Retinal sections from heterozygous and homozygous mutant mice stained with anti-Ttc8 antibodies. Signal is observed within the connecting cilium region of the photoreceptor outer segments of heterozygous animals; no signal is detected in null mice. (D) Loss of TTC8 results in mild tubular dilation in the deep cortex of the kidney. Sections from 8-month-old heterozygous and homozygous mutant mice were stained with hematoxylin and eosin and imaged by bright-field microscopy.

Copyright

This image is from Tadenev AL, Proc Natl Acad Sci U S A 2011 Jun 21;108(25):10320-5. Copyright 2011 National Academy of Sciences, U.S.A. J:173465

Associated Alleles

Symbol	Name
Ttc8^tm1Reed	tetratricopeptide repeat domain 8; targeted mutation 1, Randall R Reed

Associated Genotypes

Allelic Composition	Genetic Background
Ttc8^tm1Reed/Ttc8^tm1Reed	involves: 129