Phenotype Image Detail

Image

Caption

Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum mutation causes haploid spermatid defect. Tdrd7^tm1.1Chum/Tdrd7⁺ (Tdrd7^+/-) and Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum (Tdrd7^-/-) testes at 10 weeks. Homozygous mutant testes (675 mg, n=12) are smaller than control heterozygous testes (10910 mg, n=12) (P<0.01). (C-G) Hematoxylin and eosin (H&E) stained sections of heterozygous control (C and E) and homozygous mutant (D, F, and G) testes at 2 months. The arrowheads (G) mark homozygous mutant spermatids with aberrant nuclear morphologies. rs, round spermatids; sc, spermatocytes. (H and I) TUNEL staining (red) of apoptotic cells in control heterozygotes (H) and homozygous mutant (I) testis sections counterstained with a Hoechst dye (blue). (J) Quantification of apoptotic cells in heterozygous and homozygous mutant testes (means and SE from 50 seminiferous tubule cross-sections, P<0.01, Student's t test). (K and L) Acrosome staining [peanut agglutinin (PNA), red] of control (K) and homozygous mutant (L) spermatids counterstained with a Hoechst dye (blue). In homozygous mutant spermatids (L), the acrosomes were severely fragmented (arrowheads). (Scale bars: B, 1 mm; C and D, 50 um; E-I, 25 um; K and L, 10 um.)

Copyright

This image is from Tanaka T, Proc Natl Acad Sci U S A 2011 Jun 28;108(26):10579-84. Copyright 2011 National Academy of Sciences, U.S.A. J:173550

Associated Alleles

Symbol	Name
Tdrd7^tm1.1Chum	tudor domain containing 7; targeted mutation 1.1, Shinichiro Chuma

Associated Genotypes

Allelic Composition	Genetic Background
Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum	involves: 129S6/SvEvTac * C57BL/6