Phenotype Image Detail

Image

Caption

Tdrd6^tm1Chum/Tdrd6^tm1Chum and Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum null phenotypes. (A-H) Immunostain of heterozygous (A, C, E, and G) and Tdrd6^tm1Chum/Tdrd6^tm1Chum (B, D, F, and H) testis sections for TNP1 (A and B), TNP2 (C and D), PRM1 (E and F), and PRM2 (G and H) counterstained with a Hoechst dye (blue). Tdrd6^tm1Chum/Tdrd6^tm1Chum spermatids express transition proteins and protamines. Insets are higher magnification views showing nuclear localization of the proteins. (I and J) Anti-PRM1 and TNP2 double immunostaining of heterozygous (I) and Tdrd6^tm1Chum/Tdrd6^tm1Chum (J) elongating spermatids. Note that TNP2 in Tdrd6^tm1Chum/Tdrd6^tm1Chum spermatids shows aberrant cytoplasmic aggregations (J, arrowheads) in addition to nuclear localization. (K-N) Immunostaining of heterozygous (K), Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum (L), heterozygous (M), and Tdrd6^tm1Chum/Tdrd6^tm1Chum (N) testis sections for germ-cell-specific nuclear antigen (GENA, red). Spermatogonia were normally seen in Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum and Tdrd6^tm1Chum/Tdrd6^tm1Chum testes (arrowheads). (O-R) Immunostaining of heterozygous (O), Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum (P), heterozygous (Q), and Tdrd6^tm1Chum/Tdrd6^tm1Chum (R) testis sections for SYCP3 (green). Axial elements in meiotic spermatocytes were normally observed in Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum and Tdrd6^tm1Chum/Tdrd6^tm1Chum mutants. (AA and AB) H&E-stained sections of heterozygous (AA) and Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum (AB) eyes (1 year). The eye lenses are indicated by open arrowheads. The region marked with an asterisk shows a cataract. There is another aberrant fiber cell mass adjoining the Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum lens (AB). (Scale bars: A-H, 25 um; I-R, 10 um; AA and AB, 500 um.)

Copyright

This image is from Tanaka T, Proc Natl Acad Sci U S A 2011 Jun 28;108(26):10579-84. Copyright 2011 National Academy of Sciences, U.S.A. J:173550

Associated Alleles

Symbol	Name
Tdrd6^tm1Chum	tudor domain containing 6; targeted mutation 1, Shinichiro Chuma
Tdrd7^tm1.1Chum	tudor domain containing 7; targeted mutation 1.1, Shinichiro Chuma

Associated Genotypes

Allelic Composition	Genetic Background
Tdrd7^tm1.1Chum/Tdrd7^tm1.1Chum	involves: 129S6/SvEvTac * C57BL/6
Tdrd6^tm1Chum/Tdrd6^tm1Chum	involves: 129S4/SvJae * C57BL/6