Phenotype Image Detail

Image

Caption

Slc39a5^tm1Gka/Slc39a5^tm1Gka Tg(Cela1-cre/ERT)1Lgdn/0 (Zip 5^PancKO) mice exhibit increased sensitivity to zinc-induced acute pancreatitis. Two weeks after the last tamoxifen injection, control (Control) littermates and mutants (n = 5) were given an I.P. injection of zinc sulfate (12.5 mg zinc/kg body weight) and 48 hours later pancreata were harvested and paraffin sections were prepared and stained with hematoxylin and eosin (panels A-D) or stained for SLC39A5 using immunohistochemistry (panels E and F). Panels A and B represent control mice whereas panels C and D represent mutant mice. Dark brown deposits on the basolateral surfaces of acinar cells indicate positive staining. Black arrowheads in panel F demarcate large cytoplasmic vaculoles found in acinar cells of mutant mice in response to zinc. Panels A-D are photographed at 200X magnification and panels E and F are photographed at 1000X magnification.

Copyright

This image is from Geiser J, PLoS One 2013;8(11):e82149, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:209760

Associated Alleles

Symbol	Name
Slc39a5^tm1Gka	solute carrier family 39 (metal ion transporter), member 5; targeted mutation 1, Glen K Andrews
Tg(Cela1-cre/ERT)1Lgdn	transgene insertion 1, Craig Logsdon

Associated Genotypes

Allelic Composition	Genetic Background
Slc39a5^tm1Gka/Slc39a5^tm1Gka Tg(Cela1-cre/ERT)1Lgdn/0	involves: 129P2/OlaHsd * C57BL/6