Phenotype Image Detail

Image

Caption

Cardiac fibrosis, increased cardiomyocyte apoptosis and mitochondrial damage in Med1^tm2Jkr/Med1^tm2Jkr Tg(Myh6-cre)2182Mds/0 (csMed1^-/-) mouse heart. (A) Morphological changes in mutant mouse heart as assessed by H&E, Masson's trichrome (MT), TUNEL and DAPI staining. MERGE indicates DAPI and TUNEL overlap. (B) TUNEL positive cells in myocardium and (C) mitochondrial DNA content were determined by measuring the levels of the mitochondrial encoded gene cytochrome oxidase 1 (CO1) and the nuclear encoded 18S gene in Med1^tm2Jkr/Med1^tm2Jkr (Med1^fl/fl) and Med1^tm2Jkr/Med1^tm2Jkr Tg(Myh6-cre)2182Mds/0 (csMed1^-/-) mouse heart. (D) qPCR data represent collagen mRNA transcript in Med1^tm2Jkr/Med1^tm2Jkr and Med1^tm2Jkr/Med1^tm2Jkr Tg(Myh6-cre)2182Mds/0 mouse heart. (E&F) Represent respectively the electron micrographs of 29-day old Med1^tm2JkrMed1^tm2Jkr and Med1^tm2Jkr/Med1^tm2Jkr Tg(Myh6-cre)2182Mds/0 mouse hearts. Red arrows in F indicate lipid droplets and electron dense degenerative-bodies and black arrow points to irregularities in Z-band pattern. Results are expressed as the mean +/- SD. *p<0.05, **p<0.01.

Copyright

This image is from Jia Y, PLoS One 2016;11(8):e0160755, and is displayed under the terms of the Creative Commons Attribution 4.0 International License. J:243730

Associated Alleles

Symbol	Name
Med1^tm2Jkr	mediator complex subunit 1; targeted mutation 2, Janardan K Reddy
Tg(Myh6-cre)2182Mds	transgene insertion 2182, Michael D Schneider

Associated Genotypes

Allelic Composition	Genetic Background
Med1^tm2Jkr/Med1^tm2Jkr Tg(Myh6-cre)2182Mds/0	involves: 129P2/OlaHsd * FVB/N