Gene Expression Image Detail

Reference

Figure

ID

J:196288 Nechiporuk T, et al., Dev Biol. 2013 May 15;377(2):375-84

4

MGI:6202723

Image

Caption

(A, A') Immunofluorescent staining of E14.5 lung sections from wild-type (Ctrl) and Dlg5-/- embryos. (B-E',G,G') Immunofluorescent stainings of E15.5 lung sections from wild-type (Ctrl) and Dlg5-/- embryos. Boxed areas in C, C' are shown with split channels in D-E'. (F,G inset) Western blot analysis of total protein extracts from E15.5 wild-type (Ctrl) and Dlg5-/- lungs. (H) qRT-PCR analysis of indicated gene expression in E13.5 wild-type (Ctrl) and Dlg5-/- lungs. (I-J',N,N') Immunofluorescent staining of newborn (P0) lung sections from wild-type (Ctrl) and Dlg5-/- pups. (L) Western blot analysis of total protein extracts from E17.5 and P0 wild-type (Ctrl) and Dlg5-/- lungs. (M) qRT-PCR analysis of indicated gene expression in P0 wild-type (Ctrl) and Dlg5-/- lungs. Bar in A represents 1 mm in A, A', 0.4 mm in B, B', G, G', I, J', N, N', 100 mm in C-E'.

Copyright

Reprinted with permission from Elsevier from 10.1016/j.ydbio.2013.02.019 Dev Biol 377: 375-84, Nechiporuk T; Klezovitch O; Nguyen L; Vasioukhin V, Dlg5 maintains apical aPKC and regulates progenitor differentiation during lung morphogenesis. Copyright 2013

Associated
Assays

Label	Assay & Result Details (Gene Symbol)	Spatial Mapping
A	MGI:6203004 (Sox2)
A'	MGI:6203004 (Sox2)
B	MGI:6203006 (Pecam1)
B'	MGI:6203006 (Pecam1)
C	MGI:6203010 (Pdpn) MGI:6203011 (Sftpc)
C'	MGI:6203010 (Pdpn) MGI:6203011 (Sftpc)
D	MGI:6203011 (Sftpc)
D'	MGI:6203011 (Sftpc)
E	MGI:6203010 (Pdpn)
E'	MGI:6203010 (Pdpn)
F Dlg5	MGI:6203050 (Dlg5)
F E-cad	MGI:6203051 (Cdh1)
F Ker 8	MGI:6203052 (Krt8)
F FoxJ1	MGI:6203053 (Foxj1)
F Sox2	MGI:6203054 (Sox2)
F Sox9	MGI:6203055 (Sox9)
G	MGI:6203098 (Des) MGI:6203011 (Sftpc)
G'	MGI:6203098 (Des) MGI:6203011 (Sftpc)
G inset	MGI:6203056 (Des)
H T1a	MGI:6203058 (Pdpn)
H VEGF	MGI:6203074 (Vegfa)
H proSPC	MGI:6203059 (Sftpc)
H Pecam	MGI:6203060 (Pecam1)
I	MGI:6203075 (Aqp5)
I'	MGI:6203075 (Aqp5)
J	MGI:6203011 (Sftpc)
J'	MGI:6203011 (Sftpc)
L proSPC	MGI:6203089 (Sftpc)
L CCSP	MGI:6203090 (Scgb1a1)
L T1a	MGI:6203091 (Pdpn)
M T1a	MGI:6203092 (Pdpn)
M VEGF	MGI:6203093 (Vegfa)
M proSPC	MGI:6203094 (Sftpc)
M Pecam	MGI:6203095 (Pecam1)
N	MGI:6203096 (Scgb1a1)
N'	MGI:6203096 (Scgb1a1)